Fully enclosed portable molecular detector

Fully enclosed portable molecular detector

Build a library card box, just add samples to be tested;

Scanner, scan the bar code on the card box, the instrument automatically completes the required configuration;

control software, using Bluetooth for wireless transmission;

can meet the requirements of various sequencing platforms, without environmental requirements, portable and fast;

1 hour completed

In terms of instruments, the engineering research of a fully enclosed molecular diagnostic system in a cassette is one of the key technical aspects of this project. A stable and reliable diagnostic system is needed for downstream diagnosis. In the development of kits, fluorescent PCR- MGB probe method, design specific primers and MGB probes for target genes. Compared with ordinary TaqMan probes, the quenching group at the 3'end of the MGB probe is non-fluorescent, and the fluorescence background is lower; the MGB group allows shorter probes to still have a higher Tm value, and shorter probes can make single The detection of base mutations is more sensitive and specific.

The use of a fully enclosed cassette molecular diagnostic system eliminates cross-contamination between samples and realizes the automation of the entire process from sample preparation to result interpretation, which is the biggest innovation of this project. In addition, combining it with the strong market demand for precision medicine is another innovation